Hybrid differential defocus phase contrast
AssigneeCarl Zeiss Microscopy GmbH
InventorsLars LÖTGERING, Lars STOPPE, Markus STICKER
Life of this patent
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Abstract
Phase contrast images are calculated by digitally post-processing microscope images acquired at different angled illumination configurations and defocus values.
Claims15
A method of determining a phase contrast image based on multiple microscope images acquired using a microscope, the microscope comprising an illumination module configured for proving a switchable angled illumination of an imaging plane of the microscope, the microscope further comprising an optical system to illuminate the imaging plane and image the imaging plane onto at least one camera of the microscope,
- wherein the method comprises:
- controlling the illumination module to activate multiple angled illumination configurations, at least one of the multiple angled illumination configurations comprising a respective range of illumination directions,
- controlling the at least one camera to acquire multiple images at the multiple angled illumination configurations and at multiple defocus values, and
- combining the multiple images to determine a phase contrast image.
A method of determining a phase contrast image based on multiple microscope images acquired using a microscope, the microscope comprising an illumination module configured for proving a switchable angled illumination of an imaging plane of the microscope, the microscope further comprising an optical system to illuminate the imaging plane and image the imaging plane onto at least one camera of the microscope,
- wherein the method comprises:
- controlling the illumination module to activate multiple angled illumination configurations, the multiple angled illumination configurations having angular spectra of multiple widths,
- controlling the at least one camera to acquire multiple images at the multiple angled illumination configurations and at multiple defocus values, and
- combining the multiple images to determine a phase contrast image.
At least one processing device for determining a phase contrast image based on multiple microscope images acquired using a microscope, the microscope comprising an illumination module configured for proving a switchable angled illumination of an imaging plane of the microscope, the microscope further comprising an optical system to illuminate the imaging plane and image the imaging plane onto at least one camera of the microscope,
- wherein the at least one processing device comprises a memory storing program code and a processor configured to load the program code and execute the program code, the processor, upon executing the program code, being configured to:—control the illumination module to activate multiple angled illumination configurations, at least one of the multiple angled illumination configurations comprising a respective range of illumination directions,
- control the at least one camera to acquire multiple images at the multiple angled illumination configurations and at multiple defocus values, and
- combine the multiple images to determine a phase contrast image.
Dependencies are inferred from the claim text.