Kits, microfluidics devices, and methods for performing biotin assays
AssigneeSiemens Healthcare Diagnostics Inc.
InventorsTie Q. Wei, Zhu Teng, Xiaodong Liu, Martin Drinan
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Abstract
Kits containing a chemiluminescent detection system and microfluidics devices and methods for determining the concentration of biotin in a sample are disclosed. The kits, microfluidics devices, and methods utilize a sensitizer capable of generating singlet oxygen in its excited state and having avidin or an analog thereof directly or indirectly bound thereto, as well as a singlet oxygen-activatable chemiluminescent compound having biotin or an analog thereof directly or indirectly bound thereto.
Claims7
A kit containing a chemiluminescent detection system for determining the concentration of biotin in a sample, the kit comprising:
- (a) a composition comprising a singlet oxygen-activatable chemiluminescent compound having a biotin analog directly or indirectly bound thereto;
- and
- (b) a composition comprising a sensitizer capable of generating singlet oxygen in its excited state and having avidin or an analog thereof directly or indirectly bound thereto, wherein the avidin or analog thereof is capable of binding to biotin in the sample or to (a).
Dependencies are inferred from the claim text.