Biomarker compositions and methods of use thereof
AssigneeSiemens Healthcare Diagnostics Inc.
InventorsArejas James Uzgiris, Chris Green, Mark Baumeister
Life of this patent
Estimating the term…
Abstract
The present disclosure provides methods and kits for identifying and treating individuals at risk of or suffering from amyloid transthyretin cardiomyopathy. In general, detection or measurement of one or more biomarkers, such as TnI, PKM1, PKM2, NT-proBNP, RBP4, DCN, TIMP2, SMOC-2, NfL, or combinations thereof, assists in the identification of amyloid transthyretin cardiomyopathy. The present disclosure also provides methods for selecting patients for treatment of amyloid transthyretin cardiomyopathy, such as with transthyretin stabilizing agents.
Claims25
A method comprising:
- detecting a level of each of two or more transthyretin amyloidosis (ATTR) Biomarkers in a sample, wherein the two or more ATTR Biomarkers comprise:
- (i) troponin I (TnI),
- (ii) pyruvate kinase muscle isoform 1 (PKM1),
- (iii) pyruvate kinase muscle isoform 2 (PKM2),
- (iv) N-terminal-pro hormone B-type natriuretic peptide (NT-proBNP),
- (v) retinol binding protein 4 (RBP4),
- (vi) tissue inhibitor of metalloproteinase 2 (TIMP2),
- (vii) neurofilament light chain (NfL), or
- (viii) a combination thereof.
A method comprising:
- detecting a level of each of two or more ATTR Biomarkers in a sample, wherein the two or more ATTR Biomarkers comprise (i) TnI and PKM1, (ii) TnI and PKM2, or (iii) TnI, PKM1, and PKM2.
A method comprising:
- (a) detecting a level of each of two or more ATTR Biomarkers in a sample obtained from a subject to obtain an ATTR Biomarker profile;
- and
- (b) using the ATTR Biomarker profile to compute a ATTR Biomarker score, wherein the two or more ATTR Biomarkers comprise:
- (i) troponin I (TnI),
- (ii) pyruvate kinase muscle isoform 1 (PKM1),
- (iii) pyruvate kinase muscle isoform 2 (PKM2),
- (iv) N-terminal-pro hormone B-type natriuretic peptide (NT-proBNP),
- (v) retinol binding protein 4 (RBP4),
- (vi) tissue inhibitor of metalloproteinase 2 (TIMP2),
- (vii) neurofilament light chain (NfL), or
- (viii) a combination thereof, and
- wherein using the ATTR Biomarker profile to compute an ATTR Biomarker score comprises applying an algorithm to the ATTR Biomarker profile to compute an ATTR Biomarker score, wherein the algorithm is derived from a decision tree methodology, a neural boosted methodology, a bootstrap forest methodology, a boosted tree methodology, or a support vector machines methodology.
A composition comprising:
- (a) one or more ATTR Biomarkers, wherein the one or more ATTR Biomarkers comprise:
- (i) TnI,
- (ii) PKM1,
- (iii) PKM2,
- (iv) NT-proBNP,
- (v) RBP4,
- (vi) TIMP2,
- (vii) NfL, or
- (viii) a combination thereof;
- and
- (b) one or more anti-ATTR Biomarker agents, wherein the one or more anti-ATTR Biomarker agents comprise:
- (i) an anti-TnI agent,
- (ii) an anti-PKM1 agent,
- (iii) an anti-PKM2 agent,
- (iv) an anti-NT-proBNP agent,
- (v) an anti-RBP4 agent,
- (vi) an anti-TIMP2 agent,
- (vii) an anti-NfL agent, or
- (viii) a combination thereof.
A kit for detecting TTR-CM, said kit comprising:
- (a) one or more anti-ATTR Biomarker agents, wherein the one or more anti-ATTR Biomarker agents comprise:
- (i) an anti-TnI agent,
- (ii) an anti-PKM1 agent,
- (iii) an anti-PKM2 agent,
- (iv) an anti-NT-proBNP agent,
- (v) an anti-RBP4 agent,
- (vi) an anti-TIMP2 agent,
- (vii) an anti-NfL agent, or
- (viii) a combination thereof;
- and
- (b) instructions for use.
A kit for detecting TTR-CM, said kit comprising:
- (a) one or more anti-ATTR Biomarker agents, wherein the one or more anti-ATTR Biomarker agents comprise (i) an anti-TnI agent and an anti-PKM1 agent, (ii) an anti-TnI agent and an anti-PKM2 agent, or (iii) an anti-TnI agent, an anti-PKM1 agent, and an anti-PKM2 agent;
- and
- (b) instructions for use.
A kit comprising:
- (a) one or more anti-ATTR Biomarker agents, wherein the one or more anti-ATTR Biomarker agents comprise an anti-NT-proBNP agent, an anti-TnI agent, and an anti-RBP4 agent;
- and
- (b) instructions for use.
Dependencies are inferred from the claim text.