Solid supports for decoding methods for multiplexing assays

An assay decoding system, comprising:

• a circuit comprising at least one processor;
• a fluidic analysis chip comprising a plurality of solid supports;
• and
• at least one of a thermal source or a light source in communication with the plurality of solid supports,
• wherein the circuit activates the at least one of the thermal source or the light source to cause at least one detectable physical and/or chemical change associated with at least some of the plurality of solid supports and compares first and second encoding signals for a respective solid support in the plurality of solid supports to decode different populations of solid supports in the plurality of solid supports.