Polymer dye for detecting hydrogen peroxide and hydrogen peroxide detecting material
AssigneeCanon Kabushiki Kaisha
InventorsKeigo Mizusawa, Ryuji Higashi, Masanori Seki, Takeshi Yamamoto, Kengo Kanazaki
Life of this patent
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Abstract
A polymer dye includes at least one repeating unit represented by structural formula 7 and at least one selected from the group consisting of repeating units represented by structural formulas 2 and 3: Hydrogen peroxide present in or generated through enzymatic reaction in a biological sample can be detected by reacting with the polymer dye.
Claims11
A polymer dye comprising at least one repeating unit represented by structural formula 7 and additionally including at least one selected from the group consisting of repeating units represented by structural formulas 2 and 3:
- wherein Dye in the structural formula 7 is a light absorbing material which contains an ionic or nonionic functional group and in which the maximum value of a molar extinction coefficient at a wavelength of 400 nm to 700 nm is 104 M−1cm−1 or more, and R1 in the structural formula 2 is a hydrocarbon chain having 1 to 8 carbon atoms or a substituted or unsubstituted aromatic group.
A hydrogen peroxide detection material comprising:
- a base material;
- and
- a polymer dye bonded to the base material,
- wherein the polymer dye comprises at least one repeating unit represented by structural formula 1 and additionally including at least one selected from the group consisting of repeating units represented by structural formulas 2 and 3:
- wherein Dye in the structural formula 1 is a light absorbing material which contains an ionic or nonionic functional group and in which the maximum value of a molar extinction coefficient at a wavelength of 400 nm to 700 nm is 104 M−1cm−1 or more, and R1 in the structural formula 2 is a hydrocarbon chain having 1 to 8 carbon atoms or a substituted or unsubstituted aromatic group.
A hydrogen peroxide detection material comprising:
- a base material;
- an enzyme, which generates hydrogen peroxide by reacting with a detection target metabolite as a substrate;
- and
- a polymer dye bonded to the base material, wherein the polymer dye comprises at least one repeating unit represented by structural formula 1 and additionally including at least one selected from the group consisting of repeating units represented by structural formulas 2 and 3:
- wherein Dye in the structural formula 1 is a light absorbing material which contains an ionic or nonionic functional group and in which the maximum value of a molar extinction coefficient at a wavelength of 400 nm to 700 nm is 104 M−1cm−1 or more, and R1 in the structural formula 2 is a hydrocarbon chain having 1 to 8 carbon atoms or a substituted or unsubstituted aromatic group.
A detection kit for hydrogen peroxide contained in a biological sample, the detection kit comprising a hydrogen peroxide detecting material comprising a polymer dye bonded to a base material,
- wherein the polymer dye is a polymer dye including at least one repeating unit represented by structural formula 1 and additionally including at least one selected from the group consisting of repeating units represented by structural formulas 2 and 3:
- wherein Dye in the structural formula 1 is a light absorbing material which contains an ionic or nonionic functional group and in which the maximum value of a molar extinction coefficient at a wavelength of 400 nm to 700 nm is 104 M−1cm−1 or more, and R1 in the structural formula 2 is a hydrocarbon chain having 1 to 8 carbon atoms or a substituted or unsubstituted aromatic group.
A detection kit for a metabolite contained in a biological sample, the detection kit comprising a hydrogen peroxide detection material comprising a polymer dye bonded to a base material, and an enzyme which generates hydrogen peroxide by reacting with a detection target metabolite as a substrate,
- wherein the polymer dye is a polymer dye including at least one repeating unit represented by structural formula 1 and additionally including at least one selected from the group consisting of repeating units represented by structural formulas 2 and 3:
- wherein Dye in the structural formula 1 is a light absorbing material which contains an ionic or nonionic functional group and in which the maximum value of a molar extinction coefficient at a wavelength of 400 nm to 700 nm is 104 M−1cm−1 or more, and R1 in the structural formula 2 is a hydrocarbon chain having 1 to 8 carbon atoms or a substituted or unsubstituted aromatic group.
A detection kit for a metabolite contained in a biological sample, comprising:
- a hydrogen peroxide detection material comprising a polymer dye bonded to a base material;
- and
- a separate container in which an enzyme that generates hydrogen peroxide by reacting with the metabolite as a substrate or an aqueous solution of the enzyme is contained,
- wherein the polymer dye is a polymer dye including at least one repeating unit represented by formula 1 and additionally including at least one selected from the group consisting of repeating units represented by structural formulas 2 and 3:
- wherein Dye in the structural formula 1 is a light absorbing material which contains an ionic or nonionic functional group and in which the maximum value of a molar extinction coefficient at a wavelength of 400 nm to 700 nm is 104 M−1cm−1 or more, and R1 in the structural formula 2 is a hydrocarbon chain having 1 to 8 carbon atoms or a substituted or unsubstituted aromatic group.
A polymer dye comprising at least one repeating unit represented by formula 1 and additionally including at least one selected from the group consisting of repeating units represented by structural formulas 2 and 3:
- wherein Dye in the structural formula 1 is a light absorbing material which contains an ionic or nonionic functional group and in which the maximum value of a molar extinction coefficient at a wavelength of 400 nm to 700 nm is 104 M−1cm−1 or more, and R1 in the structural formula 2 is a hydrocarbon chain having 1 to 8 carbon atoms or a substituted or unsubstituted aromatic group, and
- wherein the polymer dye further comprises a polyvinyl alcohol skeleton having a degree of saponification of 65 mol % or more.
Dependencies are inferred from the claim text.