US 2025/0027876 A1 · DETECTING AND DIFFERENTIATING NEUROTRANSMITTERS USING ULTRAVIOLET PLASMONIC-ENGINEERED NATIVE FLUORESCENCE

Abstract

Method and apparatus for detecting and differentiating neurotransmitters using ultraviolet plasmonic-engineered native fluorescence. In one example, the method includes determining a photobleaching rate constant of a neurotransmitter-containing analyte loaded onto a plasmonic-engineered biosensor and subjected to illumination by ultraviolet light. The method further includes submitting a query containing the determined rate constant to a database including calibration data representing a plurality of different neurotransmitters and a plurality of different biosensors. In at least some examples, the queried database returns a response indicating one or more of a predicted identity of the neurotransmitter together with a corresponding confidence score, an estimated amount of the neurotransmitter in the analyte, and an estimated percentage of the neurotransmitter relative to another neurotransmitter in the analyte.

Claims20

Dependent claims

An analytical method, comprising:

determining a first rate constant based on a time series of fluorescence spectra of a biosensor having loaded thereon an analyte and subjected to illumination by ultraviolet light, the biosensor including an engineered layer of plasmonic material, the analyte including a neurotransmitter, the first rate constant corresponding to a photochemical reaction of the neurotransmitter caused by the ultraviolet light;
submitting to a database a query containing at least the determined first rate constant, the database including photobleaching calibration data representing a plurality of different neurotransmitters and a plurality of different biosensors;
and
receiving from the database a response to the submitted query.

1 more element

An analytical apparatus, comprising:

a fluorimeter configured to measure a time series of fluorescence spectra of a biosensor having loaded thereon an analyte and subjected to illumination by ultraviolet light, the biosensor including an engineered layer of plasmonic material, the analyte including a neurotransmitter, different ones of the fluorescence spectra in the time series corresponding to different respective illumination times;
and
a computing device configured to:
determine a first rate constant based on the time series, the first rate constant corresponding to a photochemical reaction of the neurotransmitter caused by the ultraviolet light;
submit to a database a query containing at least the determined first rate constant, the database including photobleaching calibration data representing a plurality of different neurotransmitters and a plurality of different biosensors;
and
display on a display device a response to the submitted query received from the database.

4 more elements

Dependencies are inferred from the claim text.

Detecting and differentiating neurotransmitters using ultraviolet plasmonic-engineered native fluorescence

Life of this patent

Detecting and differentiating neurotransmitters using ultraviolet plasmonic-engineered native fluorescence

Life of this patent

Abstract

Claims20