Transparent Embedding Solvent System and Use Thereof
OwnerHu Zhao, Woo-Ping Ge +1inventors
InventorsHu Zhao, Woo-Ping Ge, Yating Yi
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Abstract
Provided are a reagent for clearing tissue or organ, a reagent for dehydration of tissue or organ, a transparent embedding solvent system (TESOS) for transparent embedding tissue or organ, a method for clearing tissue or organ, a method for transparent embedding tissue or organ using the TESOS, and a method for microscopic imaging.
Claims30
A reagent for clearing tissue or organ, comprising organic solvent and monomer crosslinker for forming organogel,
- preferably, the tissue or organ is any of tissue or organ of the animal, including but not limited to skin, hairy, muscle, bone, nerve, brain, paw, spinal cord, vertebrae, mouse paw with skin, vertebrae with bone and muscles, spleen, liver, heart, eyeball, complete head of adult mouse, or even whole body of mouse pup.
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A reagent for dehydration of tissue or organ, comprising tert-butanol (tB) and N,N,N′,N′-Tetrakis(2-Hydroxypropyl) ethylenediamine (Quadrol),
- preferably, the reagent for dehydration of tissue or organ comprises 60-80% (v/v) tB and 20-40% Quadrol (v/v), preferably, comprises 70% (v/v) tB and 30% (v/v) Quadrol;
- preferably, the tissue or organ is any of tissue or organ of the animal, including but not limited to skin, hairy, muscle, bone, nerve, brain, paw, spinal-cord, vertebrae, mouse paw with skin, vertebrae with bone and muscles, spleen, liver, heart, eyeball, complete head of adult mouse, or even whole body of mouse pup.
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Dependencies are inferred from the claim text.