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US 2025/0027173 A1Published application
Dna clocks capable of long-term timekeeping comprising a composition for measuring or predicting the duration of cellular events in cells
OwnerHyongbum KIM, Ji-Hye PARK +1inventors
InventorsHyongbum KIM, Ji-Hye PARK, Hanahrae LEE
Life of this patent
Estimating the term…
Abstract
Disclosed are a DNA clock capable of recording long periods of time, comprising a composition for measuring or estimating elapsed time in cells, and a method of measuring or estimating elapsed time in cells using the composition.
Claims10
A composition for measuring or estimating elapsed time in isolated cells, comprising:
- (a) a prime editor protein or a nucleic acid encoding the same;
- (b) a DNA tape comprising a disrupted monomer, which includes a disrupted protospacer adjacent motif (PAM), and active monomers, each including a PAM;
- and
- (c) a prime editing guide RNA (pegRNA) which comprises a spacer and a reverse transcription template (RTT) and recognizes the DNA tape.
1 more element
Isolated cells having introduced therein:
- (a) a nucleic acid that encodes a prime editor so that the prime editor is expressed or is planned to be expressed;
- (b) a DNA tape comprising a disrupted monomer, which comprises a disrupted PAM, and active monomers, each comprising a PAM;
- and
- (c) a prime editing guide RNA (pegRNA) which comprises a spacer and a reverse transcription template (RTT) and recognizes the DNA tape.
1 more element
Dependencies are inferred from the claim text.