Methods for detecting dysfunctional nk cells in leukemia patients
AssigneesCity of Hope, The Board of Trustees of the Leland Stanford Junior University
InventorsSrividya SWAMINATHAN, Holden Maecker, Anil Kumar, Caroline Duault, Adeleh Taghi Khani
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Abstract
Provided herein are, inter alia, methods for identifying dysfunctional natural killer (NK) cells in a subject with leukemia. Provided are methods for treating leukemia, including administering to the subject an effective amount of allogeneic NK cells.
Claims36
A method of identifying dysfunctional natural killer (NK) cells in a population of NK cells obtained from a subject having leukemia, wherein the method comprises detecting in said population of NK cells:
- (a) an elevated expression level of CD56 relative to a standard control;
- (b) an elevated expression level of CD94 relative to the standard control;
- (c) an elevated ratio of CD11b−CD27− NK cells to CD11b+CD27− NK cells relative to the standard control;
- (d) the presence of CD69;
- (e) the presence of a cytokine;
- or
- (f) calcium (Ca2+) mobilization;
- thereby identifying the dysfunctional NK cells.
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A method of treating leukemia in a subject in need thereof, comprising administering to the subject an effective amount of allogeneic NK cells, wherein 50% or more of a population of NK cells obtained from the subject are dysfunctional NK cells.
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A method of treating leukemia in a subject in need thereof, comprising:
- a) identifying dysfunctional natural killer (NK) cells in a population of NK cells obtained from the subject;
- and
- b) administering to the subject an effective amount of allogeneic NK cells;
- wherein 50% or more of the population of NK cells are dysfunctional NK cells.
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A method of determining a probability of survival or relapse in a subject having leukemia, comprising identifying dysfunctional natural killer (NK) cells in a population of NK cells obtained from the subject, wherein 50% or more of the population of NK cells are dysfunctional NK cells indicates that the subject has decreased probability of survival or increased probability of relapse relative to a subject wherein less than 50% of the population of NK cells are dysfunctional NK cells.
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A method of identifying a subject susceptible to leukemia relapse, comprising identifying dysfunctional natural killer (NK) cells in a population of NK cells obtained from the subject, wherein 50% or more of the population of NK cells are dysfunctional NK cells indicates that the subject is susceptible to leukemia relapse.
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Dependencies are inferred from the claim text.