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US 2025/0027158 A1Published application
Detection of colon neoplasia by analysis of methylated dna
AssigneesExact Sciences Corporation, Mayo Foundation for Medical Education and Research
InventorsHatim T. Allawi, Michael W. Kaiser, Graham P. Lidgard, William R. Taylor, Tamara J. Sander, Abram M. Vaccaro
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Abstract
Provided herein is technology for neoplasia screening, and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of cancer, in particular, colorectal cancer.
Claims21
-98. (canceled)
A composition comprising one or more reaction mixtures comprising a plurality of complexes, the plurality of complexes comprising:
- a) at least one complex, each complex comprising a target region of a methylation marker DNA selected from the group consisting of ankyrin repeat domain 13B (ANKRD13B);
- carbohydrate sulfotransferase 2 (CHST2);
- cyclin and CBS domain divalent metal cation transport mediator 1 (CNNM1);
- dedicator of cytokinesis 2 (DOCK2);
- deltex E3 ubiquitin ligase 1 (DTX1);
- dysferlin (FERIL+);
- fermitin family member 3 (FERMT3);
- Fli-1 proto-oncogene, ETS transcription factor (FLI1);
- glutamate ionotropic receptor NMDA type subunit 2D (GRIN2D);
- junctional adhesion molecule 3 (JAM3);
- leucine rich repeat containing 4 (LRRC4);
- 5-oxoprolinase, ATP-hydrolysing (OPLAH);
- platelet derived growth factor D (PDGFD);
- cAMP-dependent protein kinase inhibitor alpha (PKIA);
- protein phosphatase 2 regulatory subunit B′gamma (PPP2R5C);
- QKI, KH domain containing RNA binding (QKI);
- Septin-9 (SEPT9);
- Scm like with four mbt domains 2 (SFMBT2);
- solute carrier family 12 member 8 (SLC12A8);
- T-box transcription factor 15 (TBX15);
- TSPY like 5 (TSPYL5);
- vav guanine nucleotide exchange factor 3 (VAV3);
- zinc finger protein 304 (ZNF304);
- zinc finger protein 568 (ZNF568);
- and zinc finger protein 671 (ZNF671), and an oligonucleotide specifically hybridized to the target region of the methylation marker DNA;
- and
- b) a complex comprising a target region of B3GALT6 DNA, and an oligonucleotide specifically hybridized to the target region of the B3GALT6 DNA.
24 more elements
A kit, comprising:
- a) at least one marker oligonucleotide, wherein at least a portion of the marker oligonucleotide specifically hybridizes to a target region of a methylation marker DNA selected from the group consisting of ANKRD13B, CHST2, CNNM1, DOCK2, DTX1, FER1L4, FERMT3, FLI1, GRIN2D, JAM3, LRRC4, OPLAH, PDGFD, PKIA, PPP 2R5C, QKI, SEPT9, SFMBT2, SLC12A8, TBX15, TSPYL5, VAV3, ZNF304, ZNF568, and ZNF671;
- and
- b) at least one reference oligonucleotide, wherein at least a portion of the reference oligonucleotide specifically hybridizes to a target region of B3GALT6 reference DNA.
Dependencies are inferred from the claim text.