Probe for targeted enrichment of nucleic acid
AssigneeNanodigmbio (Nanjing) Biotechnology Co., Ltd.
InventorsBiao WANG, Liping YU, Qiang WU
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Abstract
The present disclosure provides a novel hybrid capture probe for enrichment of a target nucleic acid sequence. Its principle is that a probe sequence is divided into three segments: a middle segment is a target sequence binding segment; a 5′ end segment of one probe can be complementarily paired with a 3′ end segment of another probe, and a 3′ end segment of one probe can be complementarily paired with a 5′ end segment of another probe. This novel probe can bind more robustly to a target sequence, and has better effects in hybrid capture and targeted enrichment at low starting amounts or small target regions (Panels) compared with traditional hybrid capture probes.
Claims20
A polyolefin porous film, having a network fiber structure;
- wherein
- when observed in a 20,000×SEM image, the polyolefin porous film has the following characteristics:
- (1) within a circle having a radius of 1000 nm, a fiber orientation R_MD of fibers having a fiber diameter of greater than 15 nm in a machine direction (MD) satisfies:
- (R_MD)2<0.8, wherein R_MD is obtained by the following formula 1:
- R_MD
- =
- ∑
- 1
- n
-
- (
- Φ
- n
- ·
- L
- n
- ·
- cos
-
- θ
- n
- )
- ∑
- 1
- n
-
- (
- Φ
- n
- ·
- cos
-
- θ
- n
- )
- ;
- Formula
-
- 1
- wherein θn is an angle between a fiber and the MD, θn is obtained counterclockwise starting from the MD leftwards, a product of a fiber length value Ln in μm and cos θn is Ln cos θn, and φn is a fiber diameter value in nm;
- and
- (2) within a circle having a radius of 1000 nm, a fiber orientation R_TD of fibers having a fiber diameter of greater than 15 nm in a transverse direction (TD) satisfies:
- (R_TD)2<0.8, wherein R_TD is obtained by the following formula 2:
- R_TD
- =
- ∑
- 1
- n
-
- (
- Φ
- n
- ·
- L
- n
- ·
- cos
-
- β
- n
- )
- ∑
- 1
- n
-
- (
- Φ
- n
- ·
- cos
-
- β
- n
- )
- ;
- Formula
-
- 2
- wherein βn is an angle between a fiber and the TD, βn is obtained counterclockwise starting from the TD upwards, a product of a fiber length value Ln in μm and cos βn is Ln cos βn, and on is a fiber diameter value in nm.
A preparation method for a polyolefin porous film, wherein the polyolefin porous film has a network fiber structure;
- and when observed in a 20,000×SEM image, the polyolefin porous film has the following characteristics:
- (1) within a circle having a radius of 1000 nm, a fiber orientation R_MD of fibers having a fiber diameter of greater than 15 nm in a machine direction (MD) satisfies:
- (R_MD)2<0.8, wherein R_MD is obtained by the following formula 1:
- R_MD
- =
- ∑
- 1
- n
-
- (
- Φ
- n
- ·
- L
- n
- ·
- cos
-
- θ
- n
- )
- ∑
- 1
- n
-
- (
- Φ
- n
- ·
- cos
-
- θ
- n
- )
- ;
- Formula
-
- 1
- wherein θn is an angle between a fiber and the MD, θn is obtained counterclockwise starting from the MD leftwards, a product of a fiber length value Ln in θm and cos θn is Ln cos θn, and φn is a fiber diameter value in nm;
- and
- (2) within a circle having a radius of 1000 nm, a fiber orientation R_TD of fibers having a fiber diameter of greater than 15 nm in a transverse direction (TD) satisfies:
- (R_TD)2<0.8, wherein R_TD is obtained by the following formula 2:
- R_TD
- =
- ∑
- 1
- n
-
- (
- Φ
- n
- ·
- L
- n
- ·
- cos
-
- β
- n
- )
- ∑
- 1
- n
-
- (
- Φ
- n
- ·
- cos
-
- β
- n
- )
- ;
- Formula
-
- 2
- wherein βn is an angle between a fiber and the TD, βn is obtained counterclockwise starting from the TD upwards, a product of a fiber length value Ln in μm and cos βn is Ln cos βn, and on is a fiber diameter value in nm;
- and
- the preparation method comprises the following steps of:
- step 1, mixing polyolefin, a pore-forming agent and an additive, and extruding the mixture, to prepare a cast sheet;
- step 2, stretching the cast sheet to prepare an oil film, wherein during stretching, the temperature of the cast sheet itself does not exceed 140° C., and a temperature difference between different points of a temperature field in a stretching area is less than 0.5° C., and a ratio of a stretching ratio in the MD to a stretching ratio in the TD, i.e., a ratio of MD/TD stretching is:
- 0.9≤the ratio of MD/TD stretching≤1.1;
- and
- step 3, performing extraction on the oil film, and then performing heat setting treatment, wherein the ratio of the stretching ratio in the MD to the stretching ratio in the TD in the step 2 is maintained by expansion stretching treatment during the heat setting treatment.
Dependencies are inferred from the claim text.