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US 2025/0027151 A1Published application
Methods of sequencing circular template polynucleotides
AssigneeSingular Genomics Systems, Inc.
InventorsDaan Witters, Allen Lipson, Eli N. Glezer
Life of this patent
Estimating the term…
Abstract
Disclosed herein, inter alia, are complexes, kits, and efficient methods of sequencing two strands of a double stranded polynucleotide.
Claims20
-86. (canceled)
A cell comprising:
- (a) a first concatemer and a second concatemer, wherein (i) the first concatemer comprises multiple copies of a first circular polynucleotide and the second concatemer comprises multiple complementary copies of a second circular polynucleotide, and (ii) the first concatemer and second concatemer are each immobilized to a cellular component or matrix within the cell;
- (b) a first sequencing primer extension product hybridized to the first concatemer and a second sequencing primer extension product hybridized to the second concatemer, wherein each sequencing primer extension product comprises a nucleotide comprising a reversible terminator;
- and
- (c) a polymerase bound to each sequencing primer extension product.
1 more element
A tissue comprising:
- (a) a first concatemer and a second concatemer, wherein (i) the first concatemer comprises multiple copies of a first circular polynucleotide and the second concatemer comprises multiple complementary copies of a second circular polynucleotide, and (ii) the first concatemer and second concatemer are each immobilized to a cellular component or matrix within a cell of the tissue;
- (b) a first sequencing primer extension product hybridized to the first concatemer and a second primer extension product hybridized to the second concatemer, wherein each sequencing primer extension product comprises a nucleotide comprising a reversible terminator;
- and
- (c) a polymerase bound to each sequencing primer extension product.
1 more element
Dependencies are inferred from the claim text.