US 2025/0027142 A1Published application

A microsphere comprising a first material and a second material

AssigneeBlink AG

InventorsEugen ERMANTRAUT, Thomas ELLINGER, Oliver LEMUTH, Katrin STEINMETZER, Susanne KLINGNER, Lea KANITZ

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Abstract

The present invention relates to microsphere comprising a first material, preferably a first polymer, and a second material, preferably a second polymer. The present invention also relates to a liquid phase comprising a plurality of such microspheres. Furthermore, the present invention relates to a method for capturing a nucleic acid from a sample and to a method of amplifying a nucleic acid from a sample. The present invention also relates to the use of a plurality of microspheres in an assay for the detection of multiple nucleic acid analytes. Furthermore, the present invention relates to the use of a plurality of microspheres in an assay for the detection of a single nucleic acid analyte in multiple samples.

Claims20

Dependent claims

A microsphere comprising a first material and a second material, wherein the first material is capable of forming a porous hydrogel when exposed to, or comprising, an aqueous solution, and has a melting temperature in the range of from 40° C. to 90° C.;

and wherein the second material forms a network within said porous hydrogel, or wherein said second material is attached to said first material and both materials together form a network within said porous hydrogel;
wherein said second material has a pKa-value and is capable of precipitating in an aqueous environment at a pH≥said pKa-value;
wherein said network is capable of contracting when exposed to a temperature≥said melting temperature of said first material;
said microsphere further comprising at least one of a) a fluorescent label and b) magnetic particles.

1 more element

Dependencies are inferred from the claim text.