Method and kit for detecting n6-methyladenine in nucleic acid molecules

A kit, which comprises a first carbonyl compound and a nitrite;

• preferably, the nitrite is selected from sodium nitrite, potassium nitrite and a combination of the two;
• preferably, the kit further comprises boric acid or salt thereof (e.g., potassium borate);
• preferably, the kit further comprises dimethylsulfoxide (DMSO), N,N-dimethylformamide (DMF) or a combination thereof;
• preferably, the kit further comprises at least one substance selected from the group consisting of:
• p-toluenesulfonic acid, phosphoric acid, 2-(N-morpholino)ethanesulfonic acid (MES), sodium acetate, 3-(N-morpholino)propanesulfonic acid (MOPS), piperazine-1,4-diethanesulfonic acid (PIPES), 4-hydroxyethylpiperazineethanesulfonic acid (HEPPS), and tris(hydroxymethyl)aminomethane (TRIS);
• preferably, the kit further comprises at least one substance selected from the group consisting of:
• 2-(N-morpholino)ethanesulfonic acid (MES), sodium acetate, 3-(N-morpholino)propanesulfonic acid (MOPS), piperazine-1,4-diethyl sulfonic acid (PIPES), 4-hydroxyethylpiperazineethanesulfonic acid (HEPPS), and tris(hydroxymethyl)aminomethane (TRIS);
• preferably, the kit further comprises a substance selected from the group consisting of:
• a substance for preparing a 2-(N-morpholino)ethanesulfonic acid (MES) buffer solution, a substance for preparing a sodium acetate buffer solution, a substance for preparing a 3-(N-morphino)propanesulfonic acid (MOPS) buffer solution, a substance for preparing a piperazine-1,4-diethanesulfonic acid (PIPES) buffer solution, a substance for preparing a 4-hydroxyethylpiperazineethanesulfonic acid (HEPPS) buffer solution, a substance for preparing a tris(hydroxymethyl)aminomethane (TRIS) buffer solution, and any combination thereof;
• preferably, the kit further comprises a 2-(N-morpholino)ethanesulfonic acid (MES) buffer solution, a sodium acetate buffer solution, a 3-(N-morpholino)propanesulfonic acid (MOPS) buffer solution, a piperazine-1,4-diethanesulfonic acid (PIPES) buffer solution, a 4-hydroxyethylpiperazinoethanesulfonic acid (HEPPS) buffer solution or a tris(hydroxymethyl)aminomethane (TRIS) buffer solution or any combination thereof;
• preferably, the kit further comprises a substance selected from the following:
• a substance for preparing a phosphate buffer solution, a substance for preparing a triethylamine acetate (TEAA) buffer solution, and combinations of the two;
• preferably, the kit further comprises a phosphate buffer solution, a triethylamine acetate (TEAA) buffer solution or a combination thereof;
• preferably, the triethylamine acetate (TEAA) buffer solution comprises 45 to 50 v/v % formamide, and has a pH of 8 to 9;
• preferably, the phosphate buffer solution comprises 40 to 60 v/v % dimethylsulfoxide (DMSO), and has a pH of 7.1 to 8;
• preferably, the kit further comprises a reagent for mass spectrometry detection and/or DNA sequencing;
• preferably, the kit further comprises an instruction.