Multiplex cellular assays for screening and quality assessment of engineered cells
AssigneeCellanome, Inc.
InventorsTarun Kumar KHURANA, Ali AGAH, Yir-Shyuan WU, Filiz YASAR, Pier Federico GHERARDINI
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Abstract
The invention is directed to methods and systems for carrying out one or more highly multiplexed cellular assays. In some embodiments, one or more channels of a fluidic device are provided with photopolymerizable polymer precursors and cells randomly disposed on a surface, after which positions of cells are measured by a detector, hydrogel chambers are synthesized by photopolymerization to enclose individual cells, and channels are loaded with assay reagents. Assay signals indicative of desired cellular characteristics can be generated for each of the enclosed cells.
Claims30
A method of measuring cytotoxicity of an effector cell, the method comprising:
- (a) introducing the effector cell into a fluidic device;
- (b) introducing one or more target cells into the fluidic device;
- (c) introducing one or more polymer precursors into the fluidic device;
- (d) within the fluidic device, selectively polymerizing the one or more polymer precursors to generate a polymeric matrix forming a chamber that co-encapsulates the effector cell with at least one target cell of the one or more target cells by projecting light into the fluidic device with a spatial energy modulating element such that the projected light causes polymerization of the one or more polymer precursors to generate the polymeric matrix and form the chamber;
- and
- (e) measuring the cytotoxicity of the effector cell against the at least one target cell of the one or more target cells.
Dependencies are inferred from the claim text.