Methods for producing biomass rich in dha, palmitic acid and protein using a eukaryotic microorganism

A culture comprising

• (a) a lipid-producing eukaryotic microorganism comprising SEQ ID NO:
• 1, wherein SEQ ID NO:
• 1 comprises an A or G at position 52, an A or T at position 118, an A or G at position 127, a C or T at position 128, an A or G at position 186, an A or T at position 255, an A, G or T at position 261, an A or G at position 453, a C or T at position 454, an A or G at position 496, a C or T at position 497, an A or G at position 557, an A or G at position 569, an A or T at position 655, a C or T at position 684, an A or T at position 687, a G or T at position 689, a C or T at position 690, a C or T at position 691, an A, G or T at position 692, an A or T at position 695, an A or T at position 697, an A or G at position 699, a G or T at position 700, an A or G at position 701, an A or T at position 702, an A or G at position 705, a C or T at position 802, an A or C at position 915, a C or T at position 925, an A or G at position 1000, an A or T at position 1023, an A, G or T at position 1026, an A or G at position 1043, an A or G at position 1054, an A or G at position 1104, an A or G at position 1237, a G or T at position 1248, an A or G at position 1338, an A or T at position 1345, a C or T at position 1404, a C or T at position 1421, an A or G at position 1465, a C or T at position 1469, a C or T at position 1470, an A or T at position 1472, an A or T at position 1475, an A or T at position 1476, a C or T at position 1529, a C or T at position 1546, an A or G at position 1563, a C or T at position 1630, an A or T at position 1678, a C or G at position 1681, an A, C or G at position 1692, a C or G at position 1699, and a G or T at position 1704, wherein the 18S sequence has 100% identity to the sequence set forth in SEQ ID NO:
• 1, and
• (b) a heterotrophic medium that results in the lipid-producing eukaryotic microorganism having a lipid profile comprising 1% to 55% docosahexaenoic acid (C22:6n-3, DHA), 7% to 20% docosapentaenoic acid n-6 (C22:5n-6, DPAn6), 0.001% to 3.0% arachidonic acid (C20:4n-6, ARA), and 0.001% to 1.0% heptadecanoic acid (C17:0) by weight of total fatty acids.