Cell suitable for gene engineering, cell engineering and cellular medicine, and method for producing same
AssigneeLogomix, Inc.
InventorsYasunori AIZAWA, Tomoyuki OHNO
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Abstract
The present invention provides a cell suitable for gene engineering, cell engineering and cellular medicine, and a method for producing the same. The present invention provides a technique for removing repetitive sequences present in a specific gene region in two or more alleles, thereby facilitating gene targeting or sequencing in the region.
Claims25
A cell comprising genomic DNA with a deletion, wherein the deletion is of a region comprising a portion or the whole of an HLA-similar sequence-cluster region located in each of one or more chromosomal regions selected from the group consisting of chromosomal regions defined in the following (i) to (iv):
- (i) a series of regions comprising HLA-F, HLA-G, and HLA-A,
- (ii) a series of regions comprising HLA-C and HLA-B,
- (iii) a series of regions comprising HLA-DRA, HLA-DRB5, and HLA-DRB1, and
- (iv) a series of regions comprising HLA-DOA, HLA-DPA1, and HLA-DPB1,
- wherein
- the HLA-similar sequence-cluster region comprises all of HLA-encoding genes and HLA-similar sequences contained in each of the chromosomal regions, and
- the portion comprises at least a stretch of region, the stretch of region comprising 50% or more of genes selected from the group consisting of the HLA-encoding genes and HLA-similar sequences contained in each of the chromosomal regions.
A cell having a genome with a deletion of a region in all alleles of the genome, wherein the deletion comprises a region comprising a portion, or preferably the whole, of an MHC-similar sequence-cluster region of a locus encoding an MHC molecule.
Dependencies are inferred from the claim text.