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US 2025/0027113 A1Published application
Methods of introducing a foreign substance into a cell
AssigneesWaveCyte Biotechnologies LLC, Royal Melbourne Institute of Technology
InventorsSteve Wanjara, Elena Ivanova, The Hong Phong Nguyen, Tharushi Perera, Rodney Croft, Denver Linklater, Saulius Joudkazis
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Abstract
Provided herein are methods for introducing a foreign substance into a cell using high frequency electromagnetic energy. Provided are methods for delivery substances to both prokaryotic and to eukaryotic cells. Also provided are cells generated with the methods disclosed herein.
Claims31
A method for introducing a foreign substance into a cell, the method comprising:
- (a) providing a plurality of cells suspended in a first solution;
- (b) exposing the plurality of cells to an electromagnetic field with a frequency of about 6 to about 35 GHz;
- (c) allowing the temperature in the first solution to decrease;
- (d) repeating steps (b) and (c) one or more times;
- and
- (e) contacting the plurality of cells with the foreign substance,
- wherein the temperature of the first solution in steps (b) to (d) does not exceed about 37° C.
4 more elements
A method for introducing a foreign substance into a cell, the method comprising:
- (a) providing a plurality of cells suspended in a first solution;
- (b) exposing the plurality of cells to an electromagnetic field with a frequency of about 6 to about 35 GHz;
- and
- (c) contacting the plurality of cells with the foreign substance,
- wherein the temperature of the first solution in step (b) does not exceed about 37° C.
2 more elements
Dependencies are inferred from the claim text.