Phosphite dehydrogenase as a selectable marker for mitochondrial transformation
AssigneeNapigen, Inc.
InventorsNarendra Yadav, Hajime Sakai, Dilbag Multani, Cheryl Caster, Emil Orozco, Ganesh Kishore
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Abstract
The present disclosure relates to genetically modified cells containing mitochondria that have been transformed with a polynucleotide encoding a phosphite dehydrogenase enzyme, such that the cells can utilize phosphite as a phosphorus source.
Claims92
A cell comprising an edited mitochondrial genome, wherein the edited mitochondrial genome comprises an exogenous polynucleotide encoding a phosphite dehydrogenase or a biologically active fragment thereof.
A method comprising introducing into a mitochondrion of a cell, a first polynucleotide encoding a first polypeptide, wherein the first polypeptide comprises a phosphite dehydrogenase or a biologically active fragment thereof.
A method of controlling weeds, the method comprising:
- a. growing a plurality of plants in a presence of a phosphite, wherein at least one plant of the plurality of plants comprises a mitochondrion having an exogenous polynucleotide that encodes phosphite dehydrogenase or a biologically active fragment thereof, wherein the presence of the phosphite is sufficient to selectively promote growth of the at least one plant of the plurality of plants, resulting in an increased growth of the at least one plant of the plurality of plants relative to plants lacking phosphite dehydrogenase or a biologically active fragment thereof.
Dependencies are inferred from the claim text.