Polynucleotide Error Recognition Methods and Compositions

Abstract

The present disclosure relates, according to some embodiments, to systems, apparatus, compositions, kits, workflows, and/or methods that address errors in synthesizing and/or copying polynucleotide sequences. The present disclosure provides, for example, systems, apparatus, compositions, kits, workflows, and methods for detecting, reducing, and/or removing sequence errors including mismatches and/or indels. Enzyme composition may include, in some embodiments, a T4EndoVII endonuclease and a mismatch endonuclease, wherein the composition is cell-free, the composition has a temperature of 30° C. to 45° C., the composition comprises ≥1 unit of T4EndoVII per μL and ≥1 unit of μL of the mismatch endonuclease, the composition comprises ≥0.2 ng T4EndoVII per 20 μL and ≥7 ng mismatch endonuclease per 20 μL, the composition further comprises a buffering agent, and/or the mismatch endonuclease is EndoMS.

Dependencies are inferred from the claim text.