Novel esterases and uses thereof
AssigneeCarbios
InventorsGUY LIPPENS, CYRIL CHARLIER, MAHER BEN KHALED, ISABELLE ANDRE, MARC GUEROULT
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Abstract
The present invention relates to novel esterases, more particularly to esterase variants having improved activity and/or improved thermostability compared to the parent esterase of SEQ ID NO:1 or SEQ ID NO:2 and the uses thereof for degrading polyester containing material, such as plastic products. The esterases of the invention are particularly suited to degrade polyethylene terephthalate, and material containing polyethylene terephthalate.
Claims20
An esterase variant which (i) has at least 90% identity to the full length amino acid sequence set forth in SEQ ID NO:
- 1, (ii) has at least one amino acid substitution selected from V219E, N204S, N243Y, L15V/Q, D158C, T160C, R138K/D/E/L, N211F, S13L, A14Y and S206N and/or one amino acid substitution, as compared to SEQ ID NO:
- 1, at at least one amino acid position corresponding to residue selected from G7, S57, T136, E141, I169, G171, V180, A184, I185, P186, Y188, E201, R234, D249, F250, R251, H77 and L191 wherein the positions are numbered by reference to the amino acid sequence set forth in SEQ ID NO:
- 1, (iii) has a polyester degrading activity and (iv) exhibits an increased thermostability and/or an increased polyester degrading activity as compared to the esterase of SEQ ID NO:
- 1.
2-25. (canceled)
An esterase which (i) has at least 90% identity to the full length amino acid sequence set forth in SEQ ID NO:
- 2, (ii) has, as compared to SEQ ID NO:
- 2, at least one amino acid substitution selected from V219E, N204S, N243Y, L15V/Q, D158C, T160C, R138K/D/E/L, N211F, S13L, A14Y and S206N and/or one amino acid substitution, as compared to SEQ ID NO:
- 1, at least one amino acid position corresponding to residue selected from G7, S57, T136, E141, 1169, G171, V180, A184, 1185, P186, Y188, E201, R234, D249, F250, R251, H77 and L191, wherein the positions are numbered by reference to the amino acid sequence set forth in SEQ ID NO:
- 2, (iii) has a polyester degrading activity and (iv) exhibits an increased thermostability and/or an increased polyester degrading activity as compared to the esterase of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.