Engineered dna polymerase variants
AssigneeCodexis, Inc.
InventorsEricka Bermudez, David Elgart, Nikki D. Kruse, Mathew G. Miller, Vesna Mitchell, Jovana Nazor, Nandhitha Subramanian
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Abstract
The present disclosure relates to engineered DNA polymerase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered DNA polymerase polypeptides. The present disclosure also provides methods of using the engineered DNA polymerase polypeptides or compositions thereof for diagnostic and other purposes.
Claims71
An engineered DNA polymerase, or a functional fragment thereof, comprising a polypeptide sequence having at least 75%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to a reference sequence corresponding to residues 12 to 850 of SEQ ID NO:
- 2, 8, 332, or 462, or to a reference sequence corresponding to SEQ ID NO:
- 2, 8, 332, or 462, wherein the polypeptide sequence comprises one or more substitutions relative to the reference sequence corresponding to residues 12 to 850 of SEQ ID NO:
- 2, 8, 332, or 462, or to the reference sequence corresponding to SEQ ID NO:
- 2, 8, 332, or 462.
Dependencies are inferred from the claim text.