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US 2025/0027055 A1Published application
Method of culturing three-dimensional cellular tissues
AssigneeToppan Holdings Inc.
InventorYasuyuki HIRAOKA
Life of this patent
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Abstract
A method of culturing three-dimensional cellular tissues, including mixing cells with a cationic substance, an extracellular matrix component and a polyelectrolyte to obtain a mixture, collecting cells from the obtained mixture to obtain cell aggregates, seeding the cell aggregates in a culture medium stored in a culture vessel, and culturing the seeded cell aggregates without suspension to thereby obtain a three-dimensional cellular tissue.
Claims20
A method of culturing three-dimensional cellular tissues, comprising:
- mixing cells with a cationic substance, an extracellular matrix component and a polyelectrolyte such that a mixture comprising the cells, the cationic substance, the extracellular matrix component and the polyelectrolyte is obtained;
- collecting cells from the mixture comprising the cells, the cationic substance, the extracellular matrix component and the polyelectrolyte such that cell aggregates are obtained;
- seeding the cell aggregates in a culture medium stored in a culture vessel;
- and
- culturing the cell aggregates seeded in the culture medium without suspension such that a three-dimensional cellular tissue is obtained.
2 more elements
A method of producing three-dimensional cellular tissues, comprising:
- suspending a population of cells comprising second cells in a solution comprising a cationic substance, an extracellular matrix component and a polyelectrolyte such that a mixture comprising the cells, the cationic substance, the extracellular matrix component and the polyelectrolyte;
- collecting cells from the mixture comprising the cells, the cationic substance, the extracellular matrix component and the polyelectrolyte such that cell aggregates are obtained;
- placing the cell aggregates in contact with a first cellular tissue comprising first cells placed in a culture medium;
- and
- culturing the cell aggregates and the first cellular tissue without suspension such that the cell aggregates form a second cellular tissue and that a three-dimensional cellular tissue in which the second cellular tissue is in contact with the first cellular tissue is formed.
2 more elements
A three-dimensional cellular tissue, comprising:
- a first cellular tissue comprising first cells;
- and
- a second cellular tissue comprising second cells in contact with the first cellular tissue.
Dependencies are inferred from the claim text.