In vitro expansion method for cultivating memory immune cell

Abstract

An in vitro expansion method for cultivating memory immune cells is provided, and the method includes: 1) using a lymphocyte separation solution to separate immune cells from peripheral blood or single blood collection; 2) activating the immune cells obtained in step 1) with a first solution in complete culture medium; 3) after activating the immune cells, adding a second solution during culture and adjusting cell culture density at intervals; and 4) using different culture containers and comparing cell expansion folds and phenotypes to determine an optimal culture container and obtain the memory immune cells. On a basis of traditional immune cell culture medium, the disclosure optimizes a culture system (cytokine type and concentration, cell culture density and culture container) and can be applied to production as a preparation method of the memory immune cells, thus improving proportion and quantity of specific T cells.

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