Methods for differentiating cells

Abstract

A method for differentiating human pluripotent stem cells (PSCs) comprising: culturing human PSCs in the form of a high-density monolayer in a primary differentiation medium so as to generate a monolayer of anterior neuroectodermal cells (ANECs); culturing the ANECs further in the primary differentiation medium so as to convert the ANECs to eye field progenitor cells (EFPCs); contacting the EFPCs with a neural retinal induction medium comprising an inhibitor of the WNT signaling pathway, an IGF pathway activator, an FGF pathway activator, N2 and B27 to produce neural retinal progenitor cells (NRPCs); converting the NRPCs to photoreceptors (PhRs) in a PhR differentiation medium comprising any one or more of: a BMP pathway inhibitor, a TGF-β pathway inhibitor, a WNT signaling pathway inhibitor, an SHH pathway activator, a thyroid pathway activator, a retinoic acid pathway activator, an IGF pathway activator, a Notch pathway inhibitor, N2 and B27.

Dependencies are inferred from the claim text.