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US 2025/0027036 A1Published application
Reagents, reagent kits, and culture media for activating and expanding immune cells and uses thereof
AssigneeNanotein Technologies, Inc.
InventorsCurtis Daniel HODGE, Patrick Michael CARNEY, Zachary Ibrahim IMAM
Life of this patent
Estimating the term…
Abstract
Disclosed herein are reagents, reagent kits, and culture media for activating and expanding immune cells in vitro. In some embodiments, the reagents, reagent kits, and culture media comprise a Quillaja saponin. In other embodiments, the reagents, reagent kits, and culture media comprise a squalene-based emulsion. Also disclosed are methods for activating and expanding immune cells in vitro using the reagents, reagent kits, and culture media.
Claims22
-31. (canceled)
A reagent kit for activating and expanding immune cells in vitro, comprising:
- a Quillaja saponin;
- and
- an anti-CD3 and anti-CD28 T-cell activation reagent, comprising a plurality of self-assembling protein nanoparticles decorated with anti-CD3 antibodies and anti-CD28antibodies, wherein at least one of the self-assembling protein nanoparticles comprises a protein cage polypeptide assembled into a three-dimensional structure and serving as a scaffold for the anti-CD3 antibodies and the anti-CD28 antibodies.
-77. (canceled)
Dependencies are inferred from the claim text.