System and methods for combined harvest and capture of a biologic

A method for isolating a biologic product from a process fluid characterized by high cell density and/or high turbidity, comprising

• performing a capture operation by contacting the process fluid with a capture resin in a retentate fluid circuit of a filtration module for a period of time sufficient to allow binding of the biologic product to the resin;
• performing a wash operation by separating the process fluid and resin mixture into permeate and retentate fluid streams of the filtration module and recirculating the retentate fluid stream through the retentate fluid circuit for a first number of diafiltration volumes (DVs) while directing the permeate stream to a waste container outside the fluid circuit, thereby producing a clarified retentate fluid, and optionally concentrating the clarified retentate fluid in the retentate fluid circuit;
• performing an elution operation by contacting the clarified retentate fluid with a volume of elution buffer in the retentate fluid circuit for a period of time sufficient to allow disassociation of the biologic product from the resin;
• performing a harvest operation by circulating the clarified retentate fluid and resin mixture through the filtration module, thereby separating the mixture into a permeate fluid stream comprising the biologic product and a retentate stream comprising the resin;
• and
• recirculating the retentate fluid stream through the retentate fluid circuit for a second number of DVs while directing the permeate stream to a recovery container outside the fluid circuit, thereby isolating the biologic product in the recovery container.