Perfusion Bioreactor With Filtration Systems

A perfusion cell culture apparatus comprising:

• (a) a bioreactor vessel configured to receive liquid media;
• (b) a first filtration assembly in fluid communication with the bioreactor vessel, wherein the first filtration assembly comprises:
• (i) a first filtration system;
• (ii) a first harvest pump connected in series with the first filtration system, wherein the first harvest pump is configured to pump the liquid media from the bioreactor vessel through the first filtration system;
• and
• (iii) a sensor, wherein the sensor is configured to interact with the liquid media inside the first filtration assembly;
• and
• (c) a second filtration assembly configured to operate in parallel with the first filtration assembly, wherein the second filtration assembly is in fluid communication with the bioreactor vessel, and wherein the second filtration assembly comprises:
• (i) a second filtration system;
• and
• (ii) a second harvest pump connected in series with the second filtration system, wherein the second harvest pump is configured to pump the liquid media from the bioreactor vessel through the second filtration system;
• and
• (d) a controller, wherein the controller executes operations comprising:
• (i) receiving, from the sensor, information indicative of changes in temperature, dissolved oxygen, pH, pCO2, capacitance, optical density, and/or oxygen uptake rate in the first filtration system;
• (ii) determining, based on at least the received information from the sensor, changes in temperature, dissolved oxygen, pH, pCO2, capacitance, optical density, and/or oxygen uptake rate in the first filtration system;
• and
• (iii) responsive to a determination of changes in temperature, dissolved oxygen, pH, pCO2, capacitance, optical density, and/or oxygen uptake rate in the first filtration system, causing the first harvest pump to stop pumping liquid media through the first filtration system.