Functionalized well plate, methods of preparation and use thereof
AssigneeBruker Cellular Analysis, Inc.
InventorsPeter J. BEEMILLER, Alexander J. MASTROIANNI, Randall D. LOWE, JR., Yelena BRONEVETSKY
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Abstract
In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces of wells within a well plate to activate lymphocytes, including but not limited to T lymphocytes, in a controllable and reproducible manner.
Claims29
(canceled)
A method of activating T lymphocytes (T cells) comprising:
- contacting a plurality of T cells with an antigen-presenting covalently functionalized region of a surface of a well plate, wherein the antigen-presenting covalently functionalized region comprises:
- a plurality of primary activating molecular ligands, each including a major histocompatibility (MHC) molecule configured to bind to a T cell receptor (TCR) of a T cell, wherein each of the plurality of primary activating molecular ligands is linked to the surface;
- and a plurality of co-activating molecular ligands, each including a T cell receptor (TCR) co-activating molecule or an adjunct TCR activating molecule, wherein each of the co-activating molecular ligands is linked to the surface;
- and culturing the plurality of T cells in contact with the antigen-presenting covalently functionalized region of the surface, thereby converting at least a portion of the plurality of T cells to activated T cells.
A method of activating a lymphocyte, comprising:
- contacting a plurality of lymphocytes with an activating moiety-presenting covalently functionalized region of a surface of a well plate, wherein the activating moiety-presenting covalently functionalized region comprises a plurality of primary activating molecular ligands linked to the surface;
- and a plurality of co-activating molecular ligands linked to the surface;
- and culturing the plurality of lymphocytes, thereby converting at least a portion of the plurality of lymphocytes to activated lymphocytes.
Dependencies are inferred from the claim text.