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US 2025/0026797 A1Published application
Gid4 n-terminal proline binder and determining a sequence of a peptide
OwnerZvi Kelman, John P. Marino +1inventors
InventorsZvi Kelman, John P. Marino, Svetlana Pavlova Ikonomova
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Abstract
A GID4 N-terminal proline binder includes: a variant of a GID4 protein that includes a mutation, wherein the variant GID4 protein exhibits an increased binding affinity for an N-terminal proline residue as compared to a native GID4 protein.
Claims31
A GID4 N-terminal proline binder, comprising:
- a variant of a GID4 protein (SEQ ID NO:
- 1) referred to as variant GID4 protein, the variant GID4 protein comprising at least one mutation selected from the group consisting of a substitution at position 252 of SEQ ID NO:
- 1, a substitution at position 253 of SEQ ID NO:
- 1, and a substitution at position 283 of SEQ ID NO:
- 1;
- and the variant GID4 protein exhibits an increased binding affinity for an N-terminal proline residue as compared to native GID4 protein (SEQ ID NO:
- 1);
- and:
- if the mutation is only substitution at position 253 of SEQ ID NO:
- 1, the amino acid at position 253 is any amino acid except D and S;
- and
- if the mutation is substitution at position 253 of SEQ ID NO:
- 1 in combination with substitution at position 252, position 283, or positions 252 and 283, the amino acid at position 253 is any amino acid except D.
10 more elements
A process for determining a sequence of a peptide comprising:
- contacting the peptide with a variant GID4 protein, the variant GID4 protein comprising at least one mutation selected from the group consisting of a substitution at position 252 of SEQ ID NO:
- 1, a substitution at position 253 of SEQ ID NO:
- 1, and a substitution at position 283 of SEQ ID NO:
- 1;
- and the variant GID4 protein exhibits an increased binding affinity for an N-terminal proline residue as compared to a native GID4 protein (SEQ ID NO:
- 1);
- determining whether the variant GID4 protein binds to the peptide;
- and identifying the presence of an N-terminal proline in the peptide based on the binding of the variant GID4 protein to the peptide;
- and
- if the mutation is only substitution at position 253 of SEQ ID NO:
- 1, the amino acid at position 252 is any amino acid except D and S;
- and
- if the mutation is substitution at position 253 of SEQ ID NO:
- 1 in combination with substitution at position 252, position 283, or positions 252 and 283, the amino acid at position 253 is any amino acid except D.
11 more elements
Dependencies are inferred from the claim text.