Intermittent Percolation Washing
AssigneePolypeptide Laboratories Holding (PPL) AB
InventorsOlivier Ludemann-Hombourger, Renan Ravetti Duran
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Abstract
The present invention relates to a process for the displacement of compounds and solvents comprised in the liquid and solid phase of a heterogeneous liquid-solid phase reaction, such as a solid phase peptide synthesis (SPPS) or solid phase oligonucleotide synthesis (SPOS). The process is applied in a reactor comprising a liquid phase and a solid phase, to which reactor a displacement liquid is fed discontinuously while the liquid phase is removed from the reactor chamber. The invention also encompasses a process for synthesizing peptides or oligonucleotides by the application of a solid phase peptide or oligonucleotide synthesis protocol further comprising the process for the displacement of compounds.
Claims34
A process for displacing compounds comprised in the liquid phase and solid phase of reaction steps of a heterogeneous chemical reaction protocol comprising a liquid phase and a solid phase, the solid phase being present in particulate form dispersed in the liquid phase, for the formation of a target-compound synthesized by the consecutive introduction of sub-units (compounds) by repetitive (recurrent) cycles, each cycle comprising reaction steps, the process comprising providing:
- a reactor where the reaction steps occur, the reactor comprising:
- a gas phase, a liquid phase, a solid phase and means for discharging the liquid phase from the reactor without essentially removing the solid phase from the reactor;
- removing the liquid phase from the reactor;
- wherein a displacement liquid is discontinuously fed to the reactor, and wherein a layer of liquid phase is always maintained over the solid phase.
A process for displacing compounds comprised in the liquid phase and solid phase of reaction steps of a solid phase peptide synthesis reaction protocol for the formation of a target peptide synthesized by the consecutive introduction of amino acids and derivatives thereof by repetitive (recurrent) cycles, each cycle comprising reaction steps comprising a liquid phase and a solid phase, the solid phase being present in particulate form dispersed in the liquid phase, the process comprising providing:
- a tank reactor where the reaction steps occur, the reactor comprising:
- an inlet, an outlet, means for facilitating mass-transfer between the liquid phase and the solid phase, a gas phase, liquid phase, a solid phase and means for discharging the liquid phase from the reactor without essentially removing the solid phase from the reactor;
- removing the liquid phase from the reactor through the outlet;
- wherein a displacement liquid is discontinuously fed through the inlet to the reactor, and wherein a liquid phase is always maintained over the solid phase until a predetermined endpoint is reached.
A process for displacing compounds (and/or solvents) comprised in a liquid phase and solid phase of reaction steps of a solid phase peptide synthesis, the process comprising providing a reactor where the reaction steps occur, the reactor comprising a liquid phase and a solid phase, means for discharging the liquid phase from the reactor without essentially removing the solid phase from the reactor, removing the liquid phase from the reactor while displacement liquid is discontinuously (intermittently) fed to the reactor, and wherein a continuous layer of liquid phase is maintained over the solid phase.
Dependencies are inferred from the claim text.