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US 2025/0025834 A1Published application
Methods for isolation and concentration of exosomes and other extracellular vesicles
AssigneeInnovaPrep LLC
InventorsAndrew E. Page, David Goad, David S. Alburty
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Abstract
Highly efficient and rapid filtration-based methods for isolation and concentration of exosomes and extracellular vesicles from biological fluids, including urine, which utilize pretreatment, prefiltration, wash steps and isolation and concentration using the concentrating pipette are disclosed.
Claims20
A method, comprising:
- diluting a raw or stabilized urine sample with a dilution fluid to produce a diluted urine sample;
- flowing the diluted urine sample through a prefilter to remove large cells, cellular debris, protein, and protein complexes while passing extracellular vesicles therethrough;
- processing of the prefiltered, diluted urine sample using a concentrating pipette tip and a concentrating pipette instrument;
- capturing extracellular vesicles and passing other particles and molecules through to a permeate;
- and
- eluting the captured extracellular vesicles into a concentrated volume.
3 more elements
A method, comprising:
- processing a diluted urine sample using a concentrating pipette prefilter, concentrating pipette tip and concentrating pipette instrument;
- capturing extracellular vesicles and passing other particles and molecules through to a permeate;
- and
- eluting the captured extracellular vesicles into a small concentrated volume.
1 more element
Dependencies are inferred from the claim text.