Cell delivery compositions and methods of use thereof
OwnerRoss C. Wilson, Dana V. Foss +5inventors
InventorsRoss C. Wilson, Dana V. Foss, John Hunsinger, Daniel Carr, Alexander Marson, David-Huy Nguyen, Srishti U. Sahu
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Abstract
The present disclosure provides a cargo delivery fusion polypeptide comprising an endosomolytic polypeptide and a cell penetrating polypeptide; and compositions comprising the cargo delivery fusion polypeptide. The present disclosure also provides methods of delivering a cargo into a target eukaryotic cell, using a composition comprising an amphiphilic polypeptide.
Claims84
A cargo delivery fusion polypeptide comprising:
- a) an endosomolytic polypeptide;
- and
- b) a cell penetrating polypeptide,
- wherein the fusion polypeptide comprises an amino acid sequence of any one of Formulas I-VIII,
- wherein the fusion polypeptide has a length of from about 32 amino acids to about 35 amino acids, and
- wherein any two adjacent amino acids are independently linked by an amide bond or a non-amide bond.
The method of any one of claims 60-4362 wherein the cell is in vitro.
A method of delivering a DNA molecule into a eukaryotic cell, the method comprising contacting the cell with a composition comprising:
- a) an amphiphilic cargo delivery fusion polypeptide comprising:
- i) an endosomolytic polypeptide;
- and
- ii) a cell penetrating polypeptide;
- and
- b) a DNA molecule comprising a nucleotide sequence encoding an immunogenic polypeptide.
A method of delivering a ribonucleoprotein (RNP) into a eukaryotic cell, the method comprising contacting the cell with a composition comprising:
- a) an amphiphilic cargo delivery fusion polypeptide comprising:
- i) an endosomolytic polypeptide;
- and
- ii) a cell penetrating polypeptide;
- and
- b) an RNP comprising:
- i) a CRISPR-Cas effector polypeptide;
- and
- ii) a guide nucleic acid.
Dependencies are inferred from the claim text.