Compositions and methods for treating an organism for a disease caused by an infectious agent in the organism

Abstract

Disclosed are compositions and methods for treating an organism for a disease caused by an infectious agent in the organism. Preferred methods comprise the steps of determining at least one protein necessary for performance of a function the infectious agent requires for survival; determining, in DNA of the infectious agent, at least one gene that can produce mRNA that can encode the protein; determining, in each gene, at least one exon arrangement that can produce the mRNA; determining at least one target sequence in the DNA, the at least one target sequence including, as to each arrangement, at least one exon in the arrangement; determining, for each target sequence, at least one protospacer adjacent motif (PAM) site, adjacent the target sequence, that can be recognized by a nuclease capable of breaking molecular bonds of the DNA; determining, for each target sequence, at least one guide RNA (gRNA) sequence complementary to the target sequence; preparing a ribonucleoprotein (RNP) complex including the gRNA sequence and the nuclease; and administering the RNP complex to the organism.

Dependencies are inferred from the claim text.