Process of preparing mrna-loaded lipid nanoparticles
AssigneeTranslate Bio, Inc.
InventorsShrirang Karve, Frank DeRosa, Ashish Sarode, Natalia Vargas Montoya, Priyal Patel
Life of this patent
Estimating the term…
Abstract
The present invention provides an improved process for lipid nanoparticle formulation and mRNA encapsulation. In some embodiments, the present invention provides a process of encapsulating messenger RNA (mRNA) in lipid nanoparticles comprising a step of combining a solution of preformed lipid nanoparticles with lipid nanoparticles encapsulating mRNA, resulting in a composition of increased potency of mRNA encoded protein expression in vivo.
Claims21
1-25. (canceled)
A composition comprising a mixture of lipid nanoparticles encapsulating mRNA (mRNA-LNPs) and preformed lipid nanoparticles (LNPs), wherein the mRNA-LNPs and the empty LNPs comprise the same cationic lipids.
A composition comprising a mixture of lipid nanoparticles encapsulating mRNA (mRNA-LNPs) and empty lipids nanoparticles (LNPs), wherein the mRNA-LNPs and/or the empty LNPs do not contain a cationic lipid.
A method of manufacturing a composition comprising lipid nanoparticles encapsulating mRNA (mRNA-LNPs), the method comprising:
- (i) dividing a stock of preformed empty lipid nanoparticles into two portions;
- (ii) mixing a first portion of preformed empty lipid nanoparticles (LNPs) with mRNA under conditions that allow formation of mRNA-LNPs;
- (iii) combining the mRNA-LNPs formed in step (ii) with the second portion of the empty LNPs in step (i), resulting in the composition comprising mRNA-LNPs.
Dependencies are inferred from the claim text.