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US 2025/0025419 A1Published application
Process of preparing mrna-loaded lipid nano particles
AssigneeTranslate Bio, Inc.
InventorsShrirang Karve, Zarna Patel, Yi Zhang, Ashish Sarode, Rebecca L. Goldman, Frank DeRosa, Michael Heartlein
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Abstract
The present invention provides an improved process for lipid nanoparticle formulation and mRNA encapsulation. In some embodiments, the present invention provides a process of encapsulating messenger RNA (mRNA) in lipid nanoparticles comprising a step of mixing a solution of pre-formed lipid nanoparticles and mRNA at a low concentration.
Claims26
A process of encapsulating messenger RNA (mRNA) in lipid nanoparticles comprising:
- mixing a solution comprising pre-formed lipid nanoparticles and mRNA such that lipid nanoparticles encapsulating mRNA are formed,
- wherein the pre-formed lipid nanoparticles and/or the mRNA are present in the solution at a concentration of no greater than 0.5 mg/ml.
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Dependencies are inferred from the claim text.