Application of Lipid Biomarkers in Diagnosis and Early Warning of Fatty Liver in Periparturient Dairy Cows
AssigneeShandong Agricultural University
InventorsKerong SHI, Tingjun LIU, Xianpeng HOU, Xiaoran YAN, Sisi FAN, Xuan ZHANG
Life of this patent
Estimating the term…
Abstract
The present application belongs to the technical field of biology. Provided is an application of lipid biomarkers in diagnosis and early warning of fatty liver in periparturient dairy cows. 20 lipid biomarkers found in the present disclosure have diagnostic values in recognizing, early warning, and diagnosing fatty liver diseases in dairy cows at corresponding disease stages. The lipid biomarkers of the present disclosure may be used for identifying and diagnosing the dairy cows with fatty liver.
Claims18
A lipid in diagnosis and early warning of fatty liver in periparturient dairy cows as a biomarker, wherein the lipid is one or more lipids of the following (1)-(8):
- (1) Ceramide (Cer) (d18:0/22:0);
- (2) Diacylglycerol (DAG) (34:1);
- (3) ether Phosphatidyl Ethanolamine (ePE) (36:4);
- (4) Lysophosphatidylcholine (LPC);
- (5) Lysophosphatidylethanolamine (LPE) (20:5);
- (6) Phosphatidylcholine (PC);
- (7) Phosphatidylethanolamine (PE);
- or
- (8) Triglyceride (TG).
A method for diagnosing conditions of periparturient dairy cows, wherein the method comprises:
- detecting the fat content in the serum of the periparturient dairy cows by means of a mass spectrometry method;
- evaluating the diagnostic value of a lipid biomarker through the ROC analysis;
- and
- determining the conditions of the periparturient dairy cows in conjunction with whether the change trend of the lipid at different stages before and after is the same as the change trend in the positive controls;
- wherein the lipid biomarker is selected from one or more of the following:
- (1) Ceramide (Cer) (d18:0/22:0);
- (2) Diacylglycerol (DAG) (34:1);
- (3) ether Phosphatidyl Ethanolamine (ePE) (36:4);
- (4) Lysophosphatidylcholine (LPC);
- (5) Lysophosphatidylethanolamine (LPE) (20:5);
- (6) Phosphatidylcholine (PC);
- (7) Phosphatidylethanolamine (PE);
- or
- (8) Triglyceride (TG).
Dependencies are inferred from the claim text.