Compositions for detecting bordetella pertussis nucleic acid

A composition for determining the presence or absence of Bordetella pertussis (Bp) in a sample, said composition comprising:

• an amplification oligomer combination comprising first and second Bp-specific amplification oligomers capable of amplifying a target region of a Bp target nucleic acid, wherein the first and second Bp-specific amplification oligomers comprise, respectively, a first Bp-specific target-hybridizing sequence and a second Bp-specific target-hybridizing sequence, wherein
• (a) the first Bp-specific target-hybridizing sequence is SEQ ID NO:9, SEQ ID NO:
• 12, SEQ ID NO:45, a sequence having at least 85% identity with SEQ ID NO:
• 4, a sequence having at least 85% identity with SEQ ID NO:6, or an RNA equivalent or DNA/RNA chimeric thereof, and
• the second Bp-specific target-hybridizing sequence is SEQ ID NO:5, SEQ ID NO:
• 8, SEQ ID NO:11, SEQ ID NO:14, or SEQ ID NO:46, or an RNA equivalent or DNA/RNA chimeric thereof;
• or
• (b) the first Bp-specific target-hybridizing sequence is SEQ ID NO:18, SEQ ID NO:
• 21, or SEQ ID NO:27, or an RNA equivalent or DNA/RNA chimeric thereof, and
• the second Bp-specific target-hybridizing sequence is SEQ ID NO:20, SEQ ID NO:23, or SEQ ID NO:29, or an RNA equivalent or DNA/RNA chimeric thereof;
• and
• a Bp-specific detection probe oligomer comprising a Bp-specific detection probe target-hybridizing sequence that is from about 15 to about 35 nucleotides in length and is configured to hybridize to a target sequence contained within a Bp amplicon amplifiable by the first and second Bp-specific amplification oligomers, wherein the Bp-specific detection probe oligomer comprises a fluorescent or chemiluminescent label.