Attaching nucleic acids to a platinum electrode
AssigneesUniversite Paris-Saclay, Centre National de la Recherche Scientifique (CNRS)
InventorsJean GAMBY, Jeremy LE GALL, Djamila KECHKECHE, Claire POUJOULY
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AssigneesUniversite Paris-Saclay, Centre National de la Recherche Scientifique (CNRS)
InventorsJean GAMBY, Jeremy LE GALL, Djamila KECHKECHE, Claire POUJOULY
Estimating the term…
The invention relates to a method for attaching nucleic acids to a platinum electrode, comprising the following steps: —a step (S1) of attaching an ethylenediamine molecule to the electrode, step (S1) comprising electro-oxidation of a primary amine from the ethylenediamine molecule by cyclic voltammetry, —a step (S2) of attaching a sulfo SMCC molecule of 4-(N-maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester to the ethylenediamine molecule, —a step (S3) of attaching a nucleic acid to the sulfo SMCC molecule, the nucleic acid having been modified beforehand to comprise a thiol function, the electrode being in contact with an aqueous solution, i.e. with a solution in which the solvent is water, during steps (S1), (S2) and (S3).
A method for attaching a nucleic acid to a platinum electrode, the method comprising the following steps:
A primary electrode for detecting a target nucleic acid, the primary electrode comprising:
Dependencies are inferred from the claim text.