Back to results
US 2025/0019746 A1Published application
Methods for variant detection
OwnerJoseph DOBOSY, Caifu CHEN +2inventors
InventorsJoseph DOBOSY, Caifu CHEN, Mark Aaron BEHLKE, Garrett Richard RETTIG
Life of this patent
Estimating the term…
Abstract
The invention can be used to provide a more efficient and less error-prone method of detecting variants in DNA, such as SNPs and indels. The invention also provides a method for performing inexpensive multiplex assays. The invention also provides methods for detection of DNA sequences altered after cleavage by a targetable endonuclease, such as the CRISPR Cas9 protein from the bacterium Streptococcus pyogenes.
Claims12
A blocked-cleavable primer for rhPCR, the primer comprising:
- 5′-A-B-C-D-E-3′
- wherein
- A is optional and is a tail extension that is not complementary to a target;
- B is a sequence domain that is complementary to a target;
- C is a discrimination domain;
- D is a cleavage domain that, when hybridized to the target, is cleavable by RNase H2, and which comprises an RNA base;
- and
- E is a blocking domain that prevents extension of the primer.
5 more elements
Dependencies are inferred from the claim text.