Methods of generating self-replicating rna molecules
AssigneeReplicate Bioscience, Inc.
InventorsNathaniel Stephen WANG, Shigeki Joseph MIYAKE-STONER, Parinaz ALIAHMAD
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Abstract
The present disclosure generally relates to new processes for generating self-replicating RNA (srRNA) systems with superior expression properties. The disclosure also provides nucleic acids and recombinant cells expressing such srRNA constructs as well as pharmaceutical compositions containing the same. Further provided are compositions and methods for inducing pharmacodynamic effects in a subject and for the prevention and/or treatment of various health conditions.
Claims98
A method for identifying and/or characterizing a self-replicating RNA (srRNA) construct, the method comprising:
- a) providing a plurality of srRNA expression constructs each comprising a coding sequence for a polypeptide construct of interest (PCI) operably inserted into an alphavirus srRNA vector, wherein at least a portion of the coding sequence for the alphavirus structural proteins has been replaced with the coding sequence of the PCI;
- b) analyzing level and/or functionality of the PCIs that are expressed from the plurality of srRNA expression constructs to identify one or more candidate PCIs having a defined property;
- c) incorporating the srRNA expression constructs capable of expressing the candidate PCIs identified in (b) with at least one delivery vehicle to create a combinatorial collection of delivery systems;
- and
- d) analyzing the delivery systems for their capability to confer at least one pharmacodynamic effect in a subject to identify a srRNA expression construct capable of conferring a desired pharmacodynamic effect.
Dependencies are inferred from the claim text.