Method, kit and system for end labeling of nucleic acids
AssigneeCheng-Yao Chen
InventorsCheng-Yao CHEN, Yi-Wen CHENG
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Abstract
Provided is a method for labeling a nucleic acid, including providing a target nucleic acid to be labeled; providing a 5′-end glycosylase to react with the target nucleic acid to create an intermediate nucleic acid having an abasic site at a 5′-end; and providing an aldehyde-reactive compound carrying a detectable label for coupling with the intermediate nucleic acid to form a labeled nucleic acid with the detectable label attached at 5′-end. Also provided are a kit and a system for labeling nucleic acids.
Claims20
A kit for 5′-end labeling of a nucleic acid, comprising:
- a 5′-end glycosylase;
- and;
- an aldehyde-reactive compound carrying a detectable label.
A method for 5′-end labeling of a nucleic acid, comprising:
- providing a target nucleic acid to be labeled;
- providing a 5′-end glycosylase to react with the target nucleic acid to create an intermediate nucleic acid having an abasic site at a 5′-end of the target nucleic acid;
- and
- providing an aldehyde-reactive compound carrying a detectable label for coupling with the intermediate nucleic acid at the abasic site to form a labeled nucleic acid with the detectable label attached at the 5′-end.
A system for 5′-end labeling of nucleic acids, comprising a reaction reservoir, a liquid handling device, a temperature control unit, and a time control unit, wherein the liquid handling device is configured to transfer a 5′-end glycosylase and an aldehyde-reactive compound to react with the nucleic acids in the reaction reservoir for a period of time at a defined temperature controlled by the temperature control unit.
Dependencies are inferred from the claim text.