In pha production
OwnerLeticia R. CARRILLO-MAXinventor
Life of this patent
Estimating the term…
Abstract
An embodiment of the present disclosure provides an isolated nucleic acid molecule comprising a sequence encoding a protein having a PhaP1 domain and a HlyA domain, wherein the HlyA domain differs from the wild-type HlyA domain of E. coli (SEQ ID NO.: 1) SEQ ID NO. 1: LAYGSQGDLNPLINEISKIISAAGSFDVKEERTAAS LLQLSGNASDFSYGRNSITLTTSA in at least the wild-type amino acid residue S (serine) at position 25 (underlined above) is replaced with the amino acid residue N (asparagine).
Claims20
An isolated nucleic acid molecule comprising a sequence encoding a protein having a PhaP1 domain and a HlyA domain, wherein the HlyA domain differs from the wild-type HlyA domain of E. coli (SEQ ID NO.:
- 1)
- SEQ ID NO. 1:
- LAYGSQGDLNPLINEISKIISAAGSFDVKEERTAAS
- LLQLSGNASDFSYGRNSITLTTSA
- in at least the wild-type amino acid residue S (serine) at position 25 (underlined above) is replaced with the amino acid residue N (asparagine).
An isolated nucleic acid molecule comprising a sequence encoding a protein having a PhaP1 domain and a HlyA domain, wherein the PhaP1 domain differs from the wild-type Ralstonia eutropha sequence (SEQ ID NO:3)
- SEQ ID NO:
- 3:
- MILTPEQVAAAQKANLETLFGLTTKAFEGVEKLVELNLQVVKTSF
- AEGVDNAKKALSAKDAQELLAIQAAAVQPVAEKTLAYTRHLYEIA
- SETQSEFTKVAEAQLAEGSKNVQALVENLAKNAPAGSESTVAIVK
- SAISAANNAYESVQKATKQAVEIAETNF
- in at least one or more of the following respects:
- the presence of amino acid residue N at position 24
- the presence of amino acid residues ATL at positions 43 to 45
- the presence of amino acid residue N at position 48
- the presence of amino acid residue E at position 50
- the presence of amino acid residue L at position 67
- the presence of amino acid residue L at position 71
- the presence of amino acid residue V at position 79
- the presence of amino acid residue S at position 83
- the presence of amino acid residue A at position 95
- the presence of amino acid residue I at position 105
- the presence of amino acid residue D at position 117
- the presence of amino acid residue V at position 119
- the presence of amino acid residue A at position 130
- the presence of amino acid residue L at position 133
- the presence of amino acid residue V at position 138
- the presence of amino acid residue S at position 161.
Dependencies are inferred from the claim text.