Methods And Systems For Producing A Protein Of Interest In A Plant
AssigneeImmunityBio, Inc.
InventorPatrick SOON-SHIONG
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Abstract
Methods and systems for producing a protein of interest within a plant or a portion of a plant are provided herein. The method includes introducing one or more nucleic acid into the plant or the portion of the plant, the nucleic acid including a nucleotide sequence encoding the protein of interest and incubating the plant or the portion of the plant under conditions that permit the expression of the nucleotide sequence encoding the protein of interest. The method also includes adding a medium amendment including a calcium carbonate source, such as aragonite, to a medium environment of the plant.
Claims20
A system for producing a protein of interest within a plant or a portion of a plant, the system comprising:
- a medium environment adapted to grow the plant, the medium environment comprising a medium amendment including a calcium carbonate source comprising aragonite, the plant including one or more nucleic acids introduced into the plant or the portion of the plant, the nucleic acid comprising a nucleotide sequence encoding the protein of interest and operatively linked to a regulatory region;
- incubation equipment adapted to incubate the plant under conditions that permit the expression of the nucleotide sequence encoding the protein of interest, thereby producing the protein of interest, the incubation equipment including at least one lighting element to supply light to the plant;
- at least one sensor associated with a plant, the at least one sensor configured to determine at least one parameter of the plant;
- a memory configured to store computer-executable instructions;
- and
- at least one processor configured to execute the instructions, wherein the instructions include:
- receiving the determined at least one parameter of the plant;
- and
- at least one of:
- controlling operation of the lighting element to modify a duration of light supplied to the plant during one or more specified daily time periods, during growth of the plant, according to the determined at least one parameter of the plant;
- controlling operation of the lighting element to modify an intensity of light supplied to the plant during growth of the plant, according to the determined at least one parameter of the plant;
- and
- controlling operation of the lighting element to modify a wavelength of light supplied to the plant during growth of the plant, according to the determined at least one parameter of the plant.
Dependencies are inferred from the claim text.