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AssigneeWhitehead Institute for Biomedical Research
InventorsRoland D. Kersten, Jing-Ke Weng
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An alternative route to moroidin-type bicyclic peptide biosynthesis is presented. Also included herein, it is reported that such moroidin-type bicyclic peptides are ribosomally synthesized and post-translationally modified peptides (RiPPs) in plants. Whereas D. moroides and C. argentea entail a previously uncharacterized DUF2775 family protein as candidate precursor peptides for moroidin biosynthesis, Japanese kerria (Kerria japonica) employs a BURP-domain protein as a precursor peptide similar to that of the recently reported lyciumin biosyntheti system. Disclosed herein are compositions and methods related to the biosynthesis of moroidin. In some embodiments of the disclosure, the moroidin peptides are synthetic. In other embodiments, the moroidin peptides are heterogenous. A skilled artisan will readily appreciate that based on the data disclosed herein that the present disclosure provides for the production of moroidins in transgenic host cells.
A method of producing one or more moroidin cyclic peptides, the method comprising:
A method of generating a library of nucleic acids encoding moroidin precursor peptides, or biologically-active fragments thereof, the method comprising constructing a plurality of vectors, each vector comprising a nucleic acid encoding a different moroidin precursor peptide, or biologically-active fragment thereof, operably linked to a heterologous promoter for expression in a host cell.
An isolated nucleic acid comprising a nucleotide sequence encoding a moroidin precursor peptide, or a biologically-active fragment thereof, operably linked to a heterologous promoter.
A library comprising a plurality of nucleic acid molecules, each nucleic acid molecule comprising a nucleotide sequence encoding a moroidin precursor peptide, or a biologically-active fragment thereof.
A method of producing one or more moroidin cyclic peptides, the method comprising:
Dependencies are inferred from the claim text.