US 2025/0019713 A1 · SHORT STATURE CORN PLANTS WITH IMPROVED SILAGE TRAITS

Abstract

A maize plant or maize plant part, and methods of making and using the same, are provided herein, wherein the maize plant or maize plant part comprises a mutant allele of an endogenous brachytic 2 (Z?r2) gene, a mutant allele of an endogenous GA20 oxidase or GA3 oxidase gene, or a transgene encoding a non-coding RNA molecule that targets an endogenous GA oxidase gene for suppression, and/or a mutant allele of an endogenous brown midrib 3 (bin3) gene. The maize plants or maize plant parts may be homozygous or heterozygous for the transgene or the mutant alleles. Additionally, the maize plant or maize plant part may be part of a silage product, where the harvest product is formed by harvesting an above-ground biomass of a population of the maize plants or a population of intercropped silage plants including such maize plants and making a silage product from the above-ground biomass.

Claims99

Dependent claims

A maize plant or maize plant part comprising:

a mutant allele of an endogenous brachytic 2 (br2) gene, or a mutant allele of an endogenous GA20 oxidase gene, or a mutant allele of an endogenous GA3 oxidase gene, or a transgene comprising a transcribable DNA sequence encoding a non-coding RNA molecule comprising a sequence that is complementary to a mRNA molecule encoding an endogenous GA20 oxidase protein or a GA3 oxidase protein, wherein the transcribable DNA sequence is operably linked to a plant expressible promoter;
and
a mutant allele of an endogenous brown midrib 3 (bm3) gene.

A method for producing maize plant seeds, comprising:

crossing a first maize plant to a second maize plant, wherein either:
(a) the first maize plant comprises a mutant allele of an endogenous brachytic 2 (br2) gene, or a mutant allele of an endogenous GA20 oxidase gene, or a mutant allele of an endogenous GA3 oxidase gene, or a transgene comprising a transcribable DNA sequence encoding a non-coding RNA molecule comprising a sequence that is complementary to a mRNA molecule encoding an endogenous GA20 oxidase protein or a GA3 oxidase protein, wherein the transcribable DNA sequence is operably linked to a plant expressible promoter;
and a mutant allele of an endogenous brown midrib 3 (bm3) gene;
(b) the first maize plant comprises a mutant allele of an endogenous br2 gene, or a mutant allele of an endogenous GA20 oxidase gene, or a mutant allele of an endogenous GA3 oxidase gene, or a transgene comprising a transcribable DNA sequence encoding a non-coding RNA molecule comprising a sequence that is complementary to a mRNA molecule encoding an endogenous GA20 oxidase protein or a GA3 oxidase protein, wherein the transcribable DNA sequence is operably linked to a plant expressible promoter, and the second maize plant comprises a mutant allele of an endogenous bm3 gene;
(c) the first maize plant comprises a mutant allele of the endogenous bm3 gene, and the second maize plant comprises a mutant allele of an endogenous br2 gene, or a mutant allele of an endogenous GA20 oxidase gene, or a mutant allele of an endogenous GA3 oxidase gene, or a transgene comprising a transcribable DNA sequence encoding a non-coding RNA molecule comprising a sequence that is complementary to a mRNA molecule encoding an endogenous GA20 oxidase protein or a GA3 oxidase protein, wherein the transcribable DNA sequence is operably linked to a plant expressible promoter;
or
(d) the second maize plant comprises a mutant allele of an endogenous br2 gene, a mutant allele of an endogenous GA20 oxidase gene, or a mutant allele of an endogenous GA3 oxidase gene, or a transgene comprising a transcribable DNA sequence encoding a non-coding RNA molecule comprising a sequence that is complementary to a mRNA molecule encoding an endogenous GA20 oxidase protein or a GA3 oxidase protein, wherein the transcribable DNA sequence is operably linked to a plant expressible promoter;
and a mutant allele of an endogenous bm3 gene;
wherein either (i) the first plant is the female plant, and second plant is the male plant;
or (ii) the second plant is the female plant, and first plant is the male plant;
and
harvesting one or more maize plant progeny seeds from the female maize plant, wherein the maize plant progeny seeds comprise the mutant allele of an endogenous br2 gene, or a mutant allele of an endogenous GA20 oxidase gene, or a mutant allele of an endogenous GA3 oxidase gene, or a transgene comprising a transcribable DNA sequence encoding a non-coding RNA molecule comprising a sequence that is complementary to a mRNA molecule encoding an endogenous GA20 oxidase protein or a GA3 oxidase protein, wherein the transcribable DNA sequence is operably linked to a plant expressible promoter;
and the mutant allele of an endogenous bm3 gene.

10 more elements

A population of maize plant seeds;

wherein the at least one maize plant seed of the population comprises a mutant allele of an endogenous brachytic 2 (br2) gene, or a mutant allele of an endogenous GA20 oxidase gene, or a mutant allele of an endogenous GA3 oxidase gene, or a transgene comprising a transcribable DNA sequence encoding a non-coding RNA molecule comprising a sequence that is complementary to a mRNA molecule encoding an endogenous GA20 oxidase protein or a GA3 oxidase protein, wherein the transcribable DNA sequence is operably linked to a plant expressible promoter;
and/or a mutant allele of an endogenous brown midrib 3 (bm3) gene.

A population of seeds comprising maize plant seeds, wherein at least a majority of the maize plant seeds of the population comprises a mutant allele of an endogenous brachytic 2 (br2) gene, a mutant allele of an endogenous GA20 oxidase gene, or a mutant allele of an endogenous GA3 oxidase gene, or a transgene comprising a transcribable DNA sequence encoding a non-coding RNA molecule comprising a sequence that is complementary to a mRNA molecule encoding an endogenous GA20 oxidase protein or a GA3 oxidase protein, wherein the transcribable DNA sequence is operably linked to a plant expressible promoter;

and a mutant allele of an endogenous brown midrib gene.

Dependencies are inferred from the claim text.

Short stature corn plants with improved silage traits

Life of this patent

Short stature corn plants with improved silage traits

Life of this patent

Abstract

Claims99