Tritirachium album proteinase k mutant and its zymogen, expression plasmid, recombinant pichia pastoris strain and method of producing the mature form of proteinase k mutant

A mutant of the Tritirachium album proteinase K zymogen containing a mutation at the C-terminus that increases the efficiency of expression in yeast cells and facilitates the inactivation of mature proteinase K under stress, the mutation being an additional Gly residue at the C-terminus.

A mutant of the Tritirachium album mature proteinase K containing a mutation at the C-terminus that increases the efficiency of expression in yeast cells and facilitates the inactivation of mature proteinase K under stress, the mutation being an additional Gly residue at the C-terminus.

The mutant according to claim 18, wherein the mutant possesses a sequence presented as SEQ ID NO. 22.

A method for obtaining a protein with Tritirachium album proteinase K activity, wherein yeast cells transformed with an expression plasmid encoding a mutant of the Tritirachium album proteinase K zymogen containing a mutation at the C-terminus, increasing the efficiency of expression in yeast cells and facilitating inactivation of mature proteinase K under stress conditions are cultured in a culture fluid, wherein the mutation is an additional Gly residue located at the C-terminus, and the obtained protein is isolated and purified from the culture fluid.