Engineered cas13 for ultrasensitive nucleic acid detection
AssigneeWilliam Marsh Rice University
InventorsXue GAO, Jie YANG, Yang GAO
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Abstract
Provided herein are engineered Cas13 proteins with enhanced collateral activity. The engineered proteins comprise RNA binding domains (RBDs) fusions within an active site-proximal loop within a higher eukaryotes and prokaryotes nucleotide-binding (HEPN) domain. Also provided are compositions comprising and methods of using the engineered Cas13 proteins to detect target nucleic acids at attomolar sensitivity.
Claims28
An engineered Cas13 enzyme comprising a binding domain fusion, wherein the engineered Cas13 enzyme has enhanced collateral activity as compared to a Cas13 enzyme.
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A method of enhancing the collateral activity of a Cas13 protein, the method comprising fusing the Cas13 protein to a binding domain, testing the collateral activity of the fusion protein, and identifying the fusion protein as having enhanced collateral activity if its collateral activity is increased as compared to a Cas13 enzyme.
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