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US 2025/0019667 A1Published application
Adenoviral helper plasmid
AssigneeForge Biologics, Inc.
InventorDavid Dismuke
Life of this patent
Estimating the term…
Abstract
The present disclosure provides improved adenoviral helper plasmids for the production of recombinant adeno-associated viruses.
Claims35
An adenoviral helper plasmid comprising a nucleotide sequence encoding:
- (a) E2a protein;
- (b) an E4 region;
- (c) a VA RNA region;
- and
- (d) an L4 region;
- wherein the adenoviral helper plasmid does not comprise a nucleotide sequence encoding one or more of:
- a Fiber protein or portion thereof;
- a L1-52/55K (Packaging Protein 3);
- and
- a Peripentonal Hexon-Associated Protein.
7 more elements
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23-25. (cancelled)
27-29. (canceled)
An adenoviral helper plasmid comprising the following adenoviral DNA sequences or regions:
- (a) E2a;
- (b) an E4 region;
- and
- (c) a VA RNA region;
- wherein the adenoviral helper plasmid does not comprise one or more of the following components:
- a Fiber or portion thereof;
- a L1-52/55K (Packaging Protein 3);
- a Peripentonal Hexon-Associated Protein;
- and
- a an L4 region.
7 more elements
An adenoviral helper plasmid having at least 80% sequence identity to any one of SEQ ID NO:
- 41-66.
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46-47. (canceled)
Dependencies are inferred from the claim text.