Methods of purifying adenovirus
OwnerThomas Linke, Matthew Thomas Aspelund +2inventors
InventorsTHOMAS LINKE, MATTHEW THOMAS ASPELUND, JESSICA PRENTICE, ADRIAN VICTOR ALLEN JOSEPH
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Abstract
Methods of purifying adenovirus that can be performed on a large scale. The methods purify adenovirus from an adenovirus-containing sample comprising or derived from a host cell population by clarifying the sample, wherein clarification comprises depth filtration followed by microfiltration; processing the clarified sample by anion exchange chromatography; and processing the anion exchange product by tangential flow filtration (TFF) to provide a TFF product.
Claims36
A method of purifying adenovirus from an adenovirus-containing sample comprising or derived from a host cell population having a cell density of at least about 4×106 cells/mL, the method comprising:
- (a) clarifying the sample to provide a clarified sample, wherein clarification comprises depth filtration followed by microfiltration;
- (b) processing the clarified sample by anion exchange chromatography to provide an anion exchange product;
- and
- (c) processing the anion exchange product by tangential flow filtration (TFF) to provide a TFF product, wherein TFF comprises ultrafiltration and diafiltration.
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